IDENTITY: Emily Witcraft
LAB TIME/DATE: 1/22/2013
Great and Negative Controls
1 . What makes there numerous washing steps in serological assessments?
To make certain that all of the extreme antibodies off, and to prevent the nonspecific brackets of the antibodies and the antigens. 2 . Explain how you knows that you had a " phony positiveвЂќ consequence. What does this mean for the rest of your outcomes?
When there is a positive result which has a negative control, and it can invalidate all of the benefits. 3. Identify how you would know that you had a " false negativeвЂќ end result. What does this mean throughout your results?
Once there is a bad result that includes a positive control, and it can invalidate all of the outcomes. Direct Fluorescent Antibody Technique
four. Why is this method a direct approach?
The antibodies include a neon dye.
5. Precisely what is an elementary body?
An contagious cell type or a compound of a number of microorganisms. six. How do primary bodies look under the fluorescent microscope?
It has some darker places, some green patches, a red color, and various in the shape. several. What do you believe would happen in case you did not repair the sample to the go with 95% ethyl liquor?
That fixes the sample, and it helps prevent the trials from getting washed away. If it wasn't used a similar would just wash off. 8. Which in turn patient(s) analyzed positive for Chlamydia?
Patients two and four.
on the lookout for. Was presently there any non-specific binding for virtually any of the samples? Explain.
Yes, sufferer three acquired one general body that had green color out of the 26 body. Ouchterlony Technique
10. What is a precipitin line?
When the antigen and the antibody are inside the optimal dimensions, crosslinking the antigen and the antibody in that case occurs, which usually forms the insoluble precipitation. 11. Precisely what is the not known antigen in the simulation?
12. Considering your outcomes, do you think that human serum albumin and bovine serum...